Optimisation of surface expression using the AIDA autotransporter
نویسندگان
چکیده
منابع مشابه
Optimisation of surface expression using the AIDA autotransporter
BACKGROUND Bacterial surface display is of interest in many applications, including live vaccine development, screening of protein libraries and the development of whole cell biocatalysts. The goal of this work was to understand which parameters result in production of large quantities of cells that at the same time express desired levels of the chosen protein on the cell surface. For this purp...
متن کاملInfluence of recombinant passenger properties and process conditions on surface expression using the AIDA-I autotransporter
Surface expression has attracted much recent interest, and it has been suggested for a variety of applications. Two such applications are whole-cell biocatalysis and the creation of live vaccines. For successful implementation of these applications there is a need for flexible surface expression systems that can yield a high level of expression with a variety of recombinant fusion proteins. The...
متن کاملSurface expression using the AIDA autotransporter: Towards live vaccines and whole-cell biocatalysis
The area of surface expression has gathered a lot of interest from research groups all over the world and much work is performed in the area. Autotransporters have been used for surface expression in Gram-negative bacteria. One of the more commonly used autotransporters is the Adhesin Involved in Diffuse Adherence (AIDA) of pathogenic Escherichia coli. The surface expression of enzymes and vacc...
متن کاملPresentation of functional organophosphorus hydrolase fusions on the surface of Escherichia coli by the AIDA-I autotransporter pathway.
We report, the surface presentation of organophosphorus hydrolase (OPH) and green fluorescent protein (GFP) fusions by employing the adhesin-involved-in-diffuse-adherence (AIDA-I) translocator domain as a transporter and anchoring motif. The surface location of the OPH-GFP fusion protein was confirmed by immunofluorescence microscopy, and protease accessibility, followed by Western blotting ana...
متن کاملAutodisplay: functional display of active beta-lactamase on the surface of Escherichia coli by the AIDA-I autotransporter.
Members of the protein family of immunoglobulin A1 protease-like autotransporters comprise multidomain precursors consisting of a C-terminal autotransporter domain that promotes the translocation of N-terminally attached passenger domains across the cell envelopes of gram-negative bacteria. Several autotransporter domains have recently been shown to efficiently promote the export of heterologou...
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ژورنال
عنوان ژورنال: Microbial Cell Factories
سال: 2011
ISSN: 1475-2859
DOI: 10.1186/1475-2859-10-72